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    Something to Chew On

    Our lives are frequently and significantly affected by food. Because we must eat to survive, many human cultures have developed with food at their very core. Through prosperous times and depression what we eat has influenced art, music, science, relationships, and more. In this fast-paced world, we often don’t take the time to consider how food gets to our plates, the importance of what and how that food is produced and the aesthetics of food requirements and food enjoyment. Often, seemingly obscure or unrelated aspects of life circle their way back to food. The goal of this podcast is to explore the complexity and nuance of food systems, celebrate the progress we have made, and debate the best ways for humans to proceed forward into the future. Join our hosts as they informally discuss these points with various contributors from Kansas State University and abroad.
    enJeff Sheldon59 Episodes

    Episodes (59)

    Tackling complex water resource challenges through collaboration and community engagement

    Tackling complex water resource challenges through collaboration and community engagement

    Water, the essence of life itself, stands as our most indispensable resource, yet often goes unappreciated. Water scarcity and sustainability pose formidable hurdles with far-reaching implications for communities, economies, and ecosystems. To navigate these challenges effectively, innovative research endeavors, advancements in agricultural technology, and collaborative partnerships across academic, governmental, and community sectors become imperative. In this episode of Something to Chew On, we highlight the work of Dr. Susan Metzger, Director of Strategic Interdisciplinary Program Development, Kansas Center for Agricultural Resources and the Environment (KCARE), Kansas Water Institute, and Institute for Digital Agricultural and Advanced Analytics (ID3A) - Engagement. At the intersection of water resource management, agricultural productivity, and interdisciplinary academic research, Dr. Metzger offers evidence-based solutions for confronting the toughest water challenges of our time.

    Something to Chew On
    enFebruary 28, 2024

    Beyond Borders: Advancing Biosecurity through Global Partnerships

    Beyond Borders: Advancing Biosecurity through Global Partnerships

    In an era of transformative global shifts—defined by evolving commodity trade patterns, the rise of online shopping, and economic challenges—a critical reevaluation of biosecurity strategies is imperative. Collaborative efforts through international partnerships, innovative approaches in business and technology, and the integration of multidisciplinary methods are essential for ensuring food security and forging novel pathways to combat pests and pathogens.

    Join us in this episode of Something to Chew On as we delve into these crucial topics with Simon McKirdy, Professor of Biosecurity, Deputy Vice Chancellor of Global Engagement, and Pro Vice Chancellor of the Harry Butler Institute at Murdoch University. A Fullbright Distinguished Chair in Life Sciences Award recipient, Dr. McKirdy's exploration of biosecurity system resiliency, coupled with his ongoing collaboration with K-State's Biosecurity Research Institute and Department of Plant Pathology, helps to establish policies and future frameworks for international coordination and global food security.

    Something to Chew On
    enJanuary 27, 2024

    The challenges of contamination and maintaining quality flour in the wheat milling industry

    The challenges of contamination and maintaining quality flour in the wheat milling industry

    Milling of wheat into flour seems like it would be pretty straight forward.  An application that simply requires running seed though a crushing process and sifting out the flour as the appropriate particle size is reached.  The reality is milling is very complex, technically challenging and requires fully interdisciplinary participation. 

    Variations in the quality of the flour from the perspective of baking performance in large scale production has always been challenging.  This is particularly true when growing seasons change the seed mix and the impact of temperature, moisture, fertilizer use, pest pressure and more changes the quality characteristics of the flour.

    The need for highly educated professionals in this area has never been greater and with the expertise of the likes of Dr. Kali Siliveru, K-State is laying the groundwork for preparing the next generation of professionals to better understand the impact of the milling process on the quality and safety of foods we eat every day.

    Something to Chew On
    enNovember 25, 2023

    Grit and Sunshine: Farming Sustainably and Eating with Intent

    Grit and Sunshine: Farming Sustainably and Eating with Intent

    Is our current food system sustainable? 

    The consumption of seasonal, locally produced food sustains nutritional value, reduces the carbon footprint, and supports the growth of local economies. In many ways we have lost our connection to food — including understanding where our food comes from, how it is produced, the comradery developed in sharing food, and why it is important to eat seasonally and with intention. 

    In this episode, we talk with Nat and Alison Bjerke-Harvey, co-owners of Piccalilli Farm in Geary County outside of Manhattan, KS. With formal training in history and environmental biology, Nat and Alison’s interest and passion for food has taken them beyond those areas of study headlong into the food system. From baking to cheese making, they have now landed squarely in the arena of sustainable farming and community building.

     Is it time to rethink what a sustainable food system is?

    Something to Chew On
    enMay 19, 2023

    Impacting the food system and changing lives in Manhattan and Riley County

    Impacting the food system and changing lives in Manhattan and Riley County

    There are so many needs when it comes to food, including the availability of food and its impact on health. How does one make a difference to meet these needs? The ability to identify needs and a passion for making a difference is what drives today’s guest, Vickie James, Coordinator of the Manhattan/Riley County Food and Farm Council.

    In this discussion, Vickie details the Food and Farm Council’s work to increase knowledge, build partnerships and provide solutions to the complex food system challenges in our local community.

    Something to Chew On
    enMay 01, 2023

    Food security challenges for university students

    Food security challenges for university students

    Food availability and food security are not always guaranteed on college campuses. Issues of food insecurity and a lack of food certainly do not end at the campus borders, but rather extend out into the city, the region and the world. If we treat these problems individually, we will fail collectively. 

    Where does student health fit into the global food system? How does nutrition connect to a college student’s overall quality of life?

    Kathleen Hatch, Morrison Family associate vice president for student well-being, joins the podcast for a conversation about K-State’s role in tackling such issues and questions, including the Cats’ Cupboard and more.

    Something to Chew On
    enApril 19, 2023

    Food and poetry: Exploring the connection between food, the five senses, creativity and language

    Food and poetry: Exploring the connection between food, the five senses, creativity and language

    Poetry provides a conduit for engaging readers in fostering feelings and understandings. Food, creativity, language, and words all play a significant role in our lives and relationships.

    In the latest episode of Something to Chew On, Traci Brimhall, professor in the Department of English at K-State and Kansas Poet Laureate, shares about her passion for poetry and food. She draws a connection between the two and shows that the humanities – including poetry – fit into so many places within the food system.

    Something to Chew On
    enApril 12, 2023

    Connecting food security and food safety, locally and beyond

    Connecting food security and food safety, locally and beyond

    How do we maintain relevant information on public health in the arena of local food producers?  Is there a connection between food security and food safety? How do we make information on mitigating these challenges available?

    In this podcast, we will discuss these issues and more with Londa Nwadike, Extension Associate Professor of Food Safety for Kansas State University and the University of Missouri. With a rich background in the international food system along with a passion for food safety and human wellbeing, Nwadike shares her insight into today’s challenges with food quality and availability.

    Something to Chew On
    enMarch 06, 2023

    Big data, interdisciplinary research and the future of agriculture

    Big data, interdisciplinary research and the future of agriculture

    The agriculture industry has advanced in many positive ways, including increases in productivity and efficiency, but the cost of those advancements could be high. Current research in agronomy is seeking ways to manage food production that will feed an ever increasing population with improved efficiency, productivity and nutritional value.   

    Ignacio Ciampitti, professor of agronomy at Kansas State University, is working with colleagues — both inside and outside of the college of agriculture — and is encouraging students to broaden their view of agronomy due to the increasing need for a multidisciplinary approach to solving problems. He joins the podcast and takes us through his passion and vision for improving the future of agronomy and food production around the world.

    Something to Chew On
    enFebruary 02, 2023

    Animal protein: Working toward the sustainable optimization of breed, feed and environmental impact

    Animal protein: Working toward the sustainable optimization of breed, feed and environmental impact

    The sustainable production of beef cattle and other animal protein is complex, and the achievement of ‘net zero’ production from an environmental perspective is challenging at best, making it difficult to know where to begin. Areas of focus in this field include using food waste streams as quality animal feed, gathering all the areas of expertise need to tackle sustainability, impacting the microbiome of feed and reducing greenhouse gases.

    Phillip Lancaster, clinical assistant professor and member of the Beef Cattle Institute at Kansas State University, joined “Something to Chew On” to discuss the work he is doing to tackle these issues.

    Something to Chew On
    enJanuary 03, 2023

    The impact and importance of feeding the world: Researching the sustainable production of grain-based food

    The impact and importance of feeding the world: Researching the sustainable production of grain-based food

    The study of cereal grains comes in many forms, from the fundamental investigation of grain components and their functionality, to processing technologies, to the development of healthy food products ready for consumption. 

    In this podcast, we welcome Sajid Alavi, professor of grain science and industry, to discuss the importance of cereal grains research in the sustainable production of grain-based foods that represents close to 70% of the world’s calorie consumption.

    Something to Chew On
    enDecember 01, 2022

    Science impacts life and life impacts science: A conversation about family, food safety and technology

    Science impacts life and life impacts science: A conversation about family, food safety and technology

    When a mechanical engineer, a microbiologist and a food scientist collide, big things happen in a very small way. The old adage, “it takes a village,” appropriately describes the importance of an interdisciplinary approach to solving food challenges.

    In this podcast we talk with Dr. Shih-Kang "Scott" Fan, professor of mechanical engineering at Kansas State, about his work on the development of a “lab on a chip” using microfluidics in the identification of STEC producing e-coli and other pathogenic organisms.

    Something to Chew On
    enOctober 25, 2022

    Food and life challenges: A discussion with Ukrainian ag economist and refugee, Antonina Broyaka

    Food and life challenges: A discussion with Ukrainian ag economist and refugee, Antonina Broyaka

    The country of Ukraine is a major worldwide producer of cereal grains and cooking oils.  The recent Russian invasion of Ukraine has put food security at risk for many parts of the world.  Our guest for this episode is Antonina Broyaka, former dean of the faculty of economics and entrepreneurship at Vinnytsia National Agrarian University in Ukraine.  Following the Russian invasion of Ukraine, Broyaka came to the United States as a refugee with her two children and is now an extension associate of the department of agricultural economics at Kansas State University. Her focus here at K-State is on the economic impact of the Russian military aggression on both Ukrainian agriculture and global food security.

    Something to Chew On
    enSeptember 15, 2022

    The future of food in Liberia: The need for agricultural development and evolution of the food system

    The future of food in Liberia: The need for agricultural development and evolution of the food system

    This summer, 24 recipients from the 2022 Mandela Washington Fellowship for Young African Leaders program joined K-State faculty in learning about leadership in civic engagement.

    In this episode, we are pleased to welcome one of those participants, Saykwayee Harmony Henry from the Republic of Liberia. Saykwayee is a mother, public speaker, entrepreneur, advocate for the rights of women and children and is the executive director at Kids Development Initiative.

    Children in Liberia go to school hungry while companies from other countries use Liberian land to grow and export crops around the world. The heart of Saykwayee’s work focuses on agriculture development and her country’s need to produce food for themselves through practical education and prioritizing healthy food production as a lifestyle and profession from a very young age.

    Something to Chew On
    enAugust 15, 2022

    How basic research can improve the food we eat

    How basic research can improve the food we eat

    This podcast episode features a team of K-State lipid researchers to help us understand food through basic lipid research and how this work improves what we eat. For this conversation, we welcome Ruth Welti, distinguished professor of biology and director of the Kansas Lipidomics Research Center; Kathrin Schrick, associate professor of biology; and Timothy Durrett, associate professor of biochemistry and molecular biophysics.

    Something to Chew On
    enJuly 12, 2022

    Destructive greenhouse gases in the food system

    Destructive greenhouse gases in the food system

    In this podcast, Jeongdae Im, Jeffrey and Joy Lessman keystone research scholar and assistant professor in the Department of Civil Engineering discusses destructive greenhouse gases in the food system. From hay bales to landfills, plastics to wastewater, the intersection of engineering and microbiological science has allowed Im to tackle multiple challenges in studying ways to mitigate the negative effects of those greenhouse gases.

    Something to Chew On
    enMay 04, 2022

    Anaerobic microorganisms importance within agricultural systems

    Anaerobic microorganisms importance within agricultural systems

    In this podcast, we talk with Prathap Parameswaran, associate professor in the Department of Civil Engineering at K-State. Parameswaran’s research focuses on the use of anaerobic microorganisms in a continuous system that produces energy and isolates materials that might be used to increase soil nutrients and more. Parameswaran discusses his approach to combating water contamination and its importance within agricultural systems.

    Something to Chew On
    enMarch 08, 2022

    The Microbiome: A discussion of life as we know it

    The Microbiome: A discussion of life as we know it

    In this podcast, we talk with Sonny Lee, assistant professor in the Division of Biology at K-State. Lee’s work touches on many areas of microbiome research and casts a wide net in laboratory studies, mining data from peers to aid in complex evaluations, and working with students and colleagues capable of critical thinking and problem solving. Lee discusses how we are in the infancy of understanding how organisms impact our health, a plant's ability to grow and everything dealing with life as we know it.

    Something to Chew On
    enFebruary 22, 2022

    Diversity is the key to Sustainability: Challenges and opportunities in the field of Weed Science

    Diversity is the key to Sustainability: Challenges and opportunities in the field of Weed Science

    Listen to our first podcast of 2022, where we discuss weed management techniques, old and new, and the tools being developed to achieve food crop yield optimization with Vipan Kumar, Ph.D., Assistant Professor in the Department of Agronomy at Kansas State University. Weeds can reduce food crop yields by more than 30%.  In this podcast, Kumar discusses the ways in which this problem might be solved when the need for food production will continue to increase, and the challenges caused by climate change create a moving target.

     

    Transcript:

    “Diversity is the key to Sustainability; Challenges and opportunities in the field of Weed Science”.   

     

    Diversity is the key for sustainability. You keep doing one thing again and again you will see a problem that we have seen in our herbicide based methods or weed control.

     

    Something to chew on is a podcast devoted to the exploration and discussion of global food systems. It's produced by the Office of Research Development at Kansas State University. I'm Maureen Olewnik, coordinator of Global Food Systems. We welcome back co host Dr. Jim Stack Professor of Plant Pathology, weeds can reduce food crop yields by more than 30%. These interlopers compete for resources including soil nutrients and water. We attempt to control weed growth through chemistry, but over time they manage to mutate, overcome, thrive, and adjust to given management techniques. So how is this problem solved when the need for food production will continue to increase and the challenges caused by climate change create a moving target. Today, we will hear more about weed management techniques old and new. And the tools being developed to achieve food crop yield optimization with Dr. Vipan Kumar, Assistant Professor in the Department of Agronomy at Kansas State University, I want to welcome you Vipan would like to before we get started in the technical side of things, just get a little background and understanding of who you are and how you got to the place that you are today as far as your professional interests go.

     

    Sure, So my name is Vipin Kumar, I'm originally from India. I did my bachelor in crop science, but finished in 2008 from Punjab Agricultural University back in India, in the state of Punjab, it's a Northwestern State in India, mainly known for wheat production and rice production. And it's very big in ag, Punjab state. So, my original goal was to help communities there, especially the farming communities to management practices they are doing so I did my bachelor there. And then I started my master actually mastering Weed Science in Pau 2008, fall 2008. But somehow I was also interested to come abroad and expand my education here in the States. So I was looking through some programs and during that time, I got to know there is a master positions open in Louisiana State. So I I applied there and I got invited and came over 2009 That was summer 2009 started my graduate research assistant with LSU, Louisiana State, Louisiana State University. So that program was specifically looking for someone who can help growers in terms of managing their irrigation water irrigation scheduling, developing some crop coefficients for the cotton prop in North East side of Louisiana. So I was based in actually a research center. It was in North East Louisiana, about five, four or five hours from the main campus Baton Rouge. So my whole research was on resource center and I got to know very few people there but I had a very excellent project to work with. So during that time, I was doing a master I got interested in Weed Science because wonderful. One of my committee member was a weed scientist. He was the superintendent with the research center and he was on my committee and glyphosate resistant Palmer Amaranth was kinda getting a lot of attention during that time in codon. So during that conversation and meeting with his students, I got interested in wheat science. So finishing master and then I started applying for PhD program. So I think during that time, there was not a whole lot of opportunity because of the economic constraints, but I found one position in Montana State University 2011 So I started my PhD 2011 in Montana State University, Bozeman, the whole my dissertation research was focused on herbicide resistant weeds, mainly Tumbleweed Kosha, looking at, you know, characterizing herbicide resistance evolution, how we can manage in terms of what strategies growers can use to control herbicide resistant Kosha in Different cropping systems. So, that was for four years I spent there and then just immediately after finishing my PhD, I started my postdoc there and two year postdoc in the same program in Montana State. So 2017 I got here at K State got this position, where I am in his as an assistant professor in Weed Science. Part of my responsibilities. I am 100% researcher. All the focus is on developing integrative weed management strategies for western Kansas. Looking at herbicide resistance evolution in weeds, what are the novel and innovative strategies we can come up for our dry land are no till dryland growers in western Kansas. So that's I have been doing last for more than 40 years in Hays, Kansas. And a little bit history on this tradition. My predecessor, Dr. Phil Stallman, he had spent 42 years on this role. He was kind of He's like one of the pioneer in herbicide resistance management in High Plains specially in dryland cropping system in Kansas. So right now leading a statewide program, research program and little bit outreach program because I've been involved with a lot of growers here are the my appointment is not extension or no extension tents, but the been doing some extension as well. So that's kind of in the nutshell, what I'm doing here. 

     

    That’s great. Okay, well, thank you so much for that overview. That's helpful in me understanding a little bit more about what it is you're doing in reading through some of the information I found on your website about what you do, there was a lot of discussion on no till and the impact of no till on managing weeds and that type of thing. Can you tell me a bit more about what that term means and how it impacts the growing period?

     

    Sure, since the dustbowl period, the soil conservation practices have been you know, taken place among growers in the main reason was those soil conservation practices were to conserve the soil and other resources for longer term because soil erosion in these areas, especially the Great Plains area, or High Plains area was pretty obvious. And because we control it was generally achieved by tillage. So folks still the ground and control the weeds in history, if you see that's like number one method it used to be and then USDA NRCS folks came up with this idea of conserving the soil not to till the ground just to preserve the soil from erosion as well as not to blow the surface soil where we have fertile soil. So, so no till is basically a concept brought up after the Dust Bowl period and got adopted by growers throughout the Great Plains. And no tillage equipments also got, you know, into the market after that like no till drills, no till planters, that growers don't have to till the ground to make the seed bed they can directly go and plant or drill their crops. And this idea or concept was achieved with the chemical weed control. So if you look at after 1940s, when the this chemical era started, like the two four D came into the market, or any other cleaning herbicide came into the market, one of those early products came into the market grower started using those and they found very convenient to kill those weeds and not till the ground. So this chemical era helped to adopt that concept of no tillage in High Plains as well as in throughout the Great Plains. So mostly what growers been doing is they don't tell the grounds they clean their fields before planting and after planting and in season crop by using chemicals and by using herbicides, so it's kind of serving to purpose they're controlling the weeds and they are also conserving the soil. Another aspect of doing no tillage is to conserve the moisture. We are in semi-arid regions our annual precip is not that great. If you look at historically we are between somewhere between 12 to 24 inches, you know depending on the place where you are in the Great Plains so doing a no tillage practice also helped conserving the moisture throughout the winters time. So whatever the snow or the moisture comes, if you don't do the ground, you know it stays there for the subsequent crop to plan and have the crop in place. There are two things basically conserving the soil and conserving the moisture that no till practice came into existence. But however, I would I also like to emphasize over the last 1015 years, what has happened is because we have relied too much on chemicals, too much on herbicides, and we are seeing evolution in weeds, they are developing or evolved resistance to these chemistries, what folks have been using in our systems. So herbicide resistant weeds have really, really become a threat to this Nortel production system and chemical industries are struggling in terms of bringing new chemistries into the market, because there is not a whole lot of investment going into bringing a new motor factions, especially from herbicide standpoint. So the dilemma is to control those herbicide resistant weeds, we need alternate strategies, alternate methods of weed control. So that's where my role kind of come into that where that fit is how we can combine different methods of weed control, including chemical or non chemical, and come up with some sort of sustainable system that can go in longer term.

     

    Yeah, if I could follow up with a question. How prevalent is this problem globally? 

     

    Herbicide resistance globally, it's, it is the number one problem for Weed Science communities as well as the grower community. Wherever folks have been using herbicides, we have been seeing increasing trend after 1980s, we have been seeing exponential increase in a number of cases of herbicide resistant weed population being reported, there is a website called Weed Science dot O R G, that documents every single case been reported to the world. And if you go to that website, you will see after 1980s, that graph has just jumped to the highest level. And it's not only one herbicide, it's basically, you know, all the available herbicide motor factions, we have reported case of resistance somewhere in the world. In the US, we are leading in that graph, country wise, in terms of herbicide resistance, the complicated issue is okay, one time a herbicide fails, for example, glyphosate. So folks start using other herbicides or other mode of action, but now been doing those things, we have been seeing multiple resistance in our weed populations. So resistance not only to one herbicide mode of action, but 23456, even six herbicide mode of action resistance in those weed species. So that's the challenge that we are having a limited options in terms of chemicals.

     

    One of the quality parameters for seed, like the grains and things like that is the number of weed seeds that are also in with the grains. Is that a significant way of moving herbicide resistant genotypes around?

     

    Yes, recently, what happened has most of our soybean, you know, most of our corn, we export to other countries. And there has been international standards in those products. And there's inert material and weed seeds are one of those standards. And recently, we have got email from our society, as well as USDA that come up with the plans how we can minimize those weed seeds in the crop seeds. Because some of the Chinese importer, they have stopped taking some of our soybean because of the big weed seeds present in those crop seeds. So it's a function of what is escaping in those crops, what is leaving in those crops at the time of harvest what you're harvesting with. And that's ultimately making those crop quality lower and making those export important difficult. And it's not only that they have they have also raised concern that hey, we don't have this, let's say big weed in China, you are sending herbicide resistant pigweed in our ways. So that's the hurdle with the growers how to sell those because the quality is lower in terms of having weed seeds in those. Those greens.

     

    Yeah, so you mentioned some, weed genotypes with resistance to five, six or more chemistries. What's the strategy then? How do you get on top of this?

     

    Yeah, I feel fortunate and excited some time that I'm in the field that where there is a lot of growth, there's a lot to do. I don't know if you have probably noticed that recently, a Weed Science area we have so many openings, so many positions coming up in industry as well as in academia and public sectors. And the reason is that we are struggling with these issues of resistance and crop weed competition in different scenarios. So, you know, considering that we are getting, you know, way back in terms of herbicide options. Industry is not coping up with the new molecules in the market. And we have more and more cases of resistance. So the shift of the research or read science research has gone to looking at non chemical strategies, what are the non chemical strategies we can bring into our system? So historically, as I said, folks used to do tillage. But in our system in Great Plains, High Plains, that's probably not a good recommendation, if you want to give folks will not like that, because we've been promoting that no till system for decades. And that is number one challenge. But in other areas, tillage is helping and it's helping those folks controlling those herbicide resistant weeds or multiple system weeds. Another approach we are looking at, what are the ecological tactics? How about the crop weed competition, how we can make our crops so competitive against weeds, that we don't have to rely too much on chemicals. One example I can give that is ecological method we are testing here is cover crops, how the cover crops can come into the system, and helps pressing those weed populations and reduce the seed bank. Again, these are not these ecological tactics don't work like chemicals, but they have a fit in our system. If we can, let's say suppress our weeds from 100 100 weeds to 70 weeds, there are still benefit having that. And you can add with the chemicals method of weed control. So that's just one example than other methods, we are looking as a non chemical methods or harvest weed seed control, that new thing is kind of getting a lot of interest among growers and researchers throughout the globe. So when I say harvest weed seed control is basically a technique when you're harvesting the crop, you have weeds in that crop, so you are harvesting the crop and you're also collecting those weed seeds. And then either you are destroying those weeds by crushing them when they're coming out of the Combine that's called harvest wheat seed destruction or you can put them as a CEF as a narrow line called chaff lining behind the combine. So this concept was brought up or discovered by a grower actually in Western Australia in a dryland wheat grower actually, just similar to what we have in western Kansas, he was struggling with the rigid ryegrass, multiple resistance to the rye grass. So what he did is he started destroying those rye grass seeds when he was harvesting wheat. So over the two, three years when he did that, he found that he reduced the seed bank, he didn't have to deal with that problem with the chemicals. So but in US or in North America, that technology has just arrived. And we are the first one in classes we have bought that destructor and Jeff minor. And we have got some USDA wants to test here in High Plains, how that's going to work in our system. I'm just giving example that those are the kind of approaches we are looking at it from the future work. Third thing which I really like to touch base is the proceeds. And that's the coming future of the Ag digital agriculture or Smart Agriculture. You can name it differently, but that's happening. So from a weed control research or weed control perspective, precision agriculture is another way to look at these problems or herbicide resistant weed problems.

     

    So how specifically does the Precision Ag is it about applying chemical where it's needed when it's needed? Is that the strategy there? Or?

     

    Yes, that there are different aspects there preseason agriculture or preseason technology is what we are, but I can envision is, you know, it can help us at least doing field mapping with to start with if we can detect early detection of herbicide resistant weed population in a farm. And then we can develop strategies accordingly. And again, then the next level of proceeds and that could be a variable rates of herbicide application or spot treatment. We don't need to spray the whole farm maybe, but just a little patch where we have herbicide resistant weeds growing. So that's where we can, you know, have precision ag tools helping us in the future if we have a good set of data, especially if you have good algorithms and good database, we can identify our pig weeds or Kosha or any other weeds in our crops, I think that can help making making your decisions or plans for weed control.

     

    Yeah, thank you. Sorry, Maureen I’ve been dominating. 

     

    No, that's okay. It was you know, as he was talking about some of the methods that they're looking at it. It took me back to my previous life. Were working in the food safety area, we focus heavily on integrated pest management, it sounds to me like the directions that you're heading now that the chemicals are not doing what they're supposed to necessarily be doing. You're looking at these integrated systems of trying to control those weed productions from a whole variety of different areas. And it may be that there are packages or approaches  that can be taken based on location based on crop type based on a variety of other things. But you will have that group of tools in your toolbox. Is that am I interpreting that correctly? 

     

    Yes, yes, you're right, you're on the same page. The things are like with this herbicide resistance management, it's all economic aspects. Economy drives these things, the farmer economy, when they are going to make their weed control decision, they're going to look at what herbicide how much it takes, what is the rate? What is the cost. And if you see, like with the roundup resistant weeds, folks have been switching to other chemistries which are more expensive, and having more other issues as well as like drift to other crops or drift to other organisms from environmental standpoint. Also, chemical control is kind of getting ahead. In terms of some folks, they don't like some chemicals because they are hitting their other organism or other crops sensitive crops. And the second is, economically Is it viable to use that chemistries, for example, you know, most of the folks most of the industry, you might notice these days, they're giving a talk having a true two or three different herbicide mode of action in a tank, they have a pre mixes available two to three actives in those pre mixes. But those are very, very expensive. Those are not cheap products to use. So the idea with the growers with the lower commodity prices, they don't want to put those high expensive herbicides at especially when you are doing in a fallow weed management, you're not getting any output or any return in those fallow fields. So to make the system more economical, you need to think about where my money is going in terms of inputs, those herbicide applications and in fallow systems grower used to spray like three, four times in the season. It's not like one application, and they're done. They used to spray three times four times. And you can imagine like 5000 acres spraying three times $10 an acre, that can multiply pretty quick. So that's where I think the folks or the weed science community is thinking to bring some of those cost effective programs or cost effective management strategies in our system that not only helps pressing this problem or suppressing these weeds, but also give benefit to the growers, and the environment and ecology or agro ecology, like a cover crops. So we are not just thinking integrating cover crops for weed suppression. But we are thinking that cover crops can help suppressing weeds. It can help you know fixing nitrogen, it can help improving the soil quality soil health. And it can also be used for grazing purpose to the animals. So there is a livestock integration as well. So we have we are thinking from a system standpoint that can help folks to be more economically viable.

     

    This next question is kind of out there as it's taking us probably outside of your major focus at this point. But I've done a little bit read a bit of reading recently on the land institute and some of the work they're doing in Salina on perennial grains. Have you looked at that at all or have any thoughts on perennial brains? And if there's any value to that and what impact it would have on what you work on?

     

    Definitely, I have not personally looked at that system yet. But I've been hearing that quite a bit. And we have a cropping system specialist here in his he's been talking one other day was giving a presentation on that side of it. But I think again, I would like to emphasize that Perennial system or perennial grain springing into our system is basically improving you know, our our ecosystem and also increasing the economic value of the products as well as the farm profitability overall. And some of the work being led by cropping system specialist here or agronomist here. Also looking at those forage species or forage annual forages or biennial forages or perennial forages as a part of the system that can integrate into our system. So, from Weed weed management side of it, I think that would be a win win situation that if that species or if those grains or perennial grains can provide that kind of weed suppression benefits what we are getting from other cover crops. I think that's what we need.

     

    So one of the reasons we care about weeds as the as we do the other pests as their impact on production and grow the crops for to feed people, we grow the crops to feed the animals that become the food that they feed people. Are there reasonable estimates of the economic impact or the yield impacts that you know, general rules of thumb? I know there, there are no exact numbers, but what what are we talking about in terms of scale of impact that we have on food production, but then also, what having herbicide resistant weeds contributes to that?

     

    Definitely, there has been several reports in different crops. And I will just highlight some of the examples here for Kosha or, or Palmer Amaranth. Those are the prevalent species here in western Kansas or central part of state, if you like, look at some of the reports on Kosha. previous reports from my previous predecessor and other colleagues in other other states, they have found Kosha is quite competitive. Irrespective of resistance, let's say there's no resistance in these species. These weed species are very, very aggressive, very invasive. They have good traits, good biological traits, to compete very well with the crops. First, you need to understand that the biology behind those weeds, that's why they're becoming more and more troublesome problem for the folks here. So in terms of yield impact, I would say Kosha, let's say you know, you leave the kosher season long infestation in a crop like that the sugar bee does the least competitive crop in among all those crops, we grow in the northern or central Great Plains by up to 95% reduction in those sucrose yield as well as the beat heels we have reported. We have seen in the literature since 1970s 1980s. Wheat 20 to 30%. Yield reduction, going to be the kosher season long infestation, when I'm saying the Kosha is like moderate densities 40 to 50 plants per square meter, if they are present, they can do that 20-30% of damage to the yield big waves, they can choke our our sorghum. So one of the worst fields I have seen in my lifetime here in western Kansas is sorghum because the folks they don't have option, there's not not a single effective option that can go with for controlling pigweed controlling Palmer Amaranth in sorghum, especially when the sorghum is above certain stage, like 30 inch tall, there's no label chemistry to go with controlling pigweed. And that's the time I start getting calls from growers, hey, our pigweed is this much our Milo is already two feet tall, can I spray Dicamba that's the off label you cannot and if you do it, you will hurt you leave you will that will cause a crop injuries that will cause reducing the grain quality. So yeah, really impact. I mean, there's a huge impact. And you can imagine now if those species are resistant, and you are putting the chemical, and they are surviving 70% of those ceilings are surviving. And you know, going up to the seed production, you can imagine that you have put the cost to control it. Plus you still have a problem, and there is a double hit there.

     

    Right. That's the double insult with resistance.

     

    Right. So yeah, that's I think that's where we need to be more proactive. And we need to think more in longer term. The growers don't think in a longer term, they think on an annual basis because their budget is running annual basis. They have like let's say 5000 acres, they have a plan for 5000 acre for one year, they don't have a plan for three year or five years. That's where the problem starts. And as I said, economy drives all these things that resistance management. And that's become really, really challenging for researcher as well as extension person to convince folks to do things they're not doing.

     

    You're talking about the aggressive nature of some of those weeds and thought just came into my mind on the genetics of those materials as any work being done at K State on the genetics of some of these weeds.

     

    Yes, yes, we have a weed physiologist, weed physiology lab in in Manhattan. There has been quite a bit of work been done. And yeah, there's all kinds of different genetic mechanisms they have found in these weed species, why they are adapting to these kinds of situations herbicide applications. One example I can give here is Kosha and Palmer Amaranth. They have developed resistance to glyphosate commonly used chemistry or herbicide in our system in Roundup Ready crops. We have seen both species Palmer and Kosha. What they do is they multiply that target gene so they have more copies of that gene with the glyphosate go and target. So what it does is instead of one copy, single gene in they have Kosha has like 10-15-20 copies of that gene. So that Are those number of copies of that gene produce more enzyme, so the chemical cannot inhibit that much enzyme. So the those plants survive those treatments. That's how they are kinda adapting to that glyphosate treatments or other mechanism recently, weed physiology lab in Manhattan, they have found these multiple resistant pigweeds, what they are doing is they have enhanced metabolism. So some of the genes involved in metabolism in those plants, they got activated, and they are just metabolizing, whatever you're spraying. So no matter what, even a new chemistries is not even existing, it can just metabolic metabolite because it's not reaching to the target gene and hitting those targets side. So that is a more fearful thing happening in the nature, that metabolism based mechanism is also evolving in weed species. And as I said, it's a function of the biology of the species like palmer amaranth, very, very diverse genetic background Kosha. Same with very diverse genetic background, a lot of gene pools, they're sitting in those, you know, individuals and they can, they can adapt, and they can evolve to any of those stresses. Among other biological feature if you read about kosher Palmer, both are highly prolific seed producers, a single kosher plant can produce hundreds of 1000s of seeds. A one female Palmer Amaranth can produce millions of seeds. So that many seed production, it has potential to infest more areas, more lands, and keep going if you don't manage them properly.

     

    Is dissemination and equipment. Problematic locally, though, going from one field to the next?

     

    Yes, yes, big weed or Palmer Amaranth. We had a meeting North Central wheat science meeting, talking with the folks from North Dakota, and South Dakota, they have started seeing palmer amaranth, it was not the case, five years back. And that's happening because of movement of equipment, movement of products, like hay movement, or even animal feed, people take the animal feed and take to the other states, and those farmer seeds go with that. And, and infest those areas. So that's kind of tricky, you know, managing those moments is very, very difficult. That's where we kind of emphasize that control those weeds in the field, so that you don't have to deal with in the products. Okay, or, or green or or equipments. For weeds like Kosha, it's a tumbleweed and doesn't need that many it can tumble miles and miles when the wind is blowing. And that's the kind of beauty of that weed species that finds new areas of infestations with the high winds, especially in the high plains, it can tumble, it's very hard to kind of contain that.

     

    How is the contaminated seeds physically removed from the grain itself? I'm sitting here trying to get in my mind if we're going to be selling to other countries, and they've got obviously a lower limit that's allowed in there. Is there some kind of assuming practice or an air movement as the heavier seed goes through? How's that done?

     

    Yeah, I don't know exactly how that will happen. Because this year, we are talking like a bulk export. And folks just take the produce from the field and sell it to the coop cooperative marketing places and I don't know how much storage they have, and it gets pretty big pretty quickly. So that's where we try to emphasize to the grower Hey, you know, if you can manage in the field, that's the best you can do. You don't let it go to the produce or to the greens I see that's where this harvest we'd see destruction is going to have a fared very well that can destroy the weed seeds don't don't don't let it go into the grains and escape folks to get the contaminated grains. And it's not only that in crops like wheat, we have a problem we have a central Kansas growers they've been dealing with awry federal MRI or CT or MRI issues. So those dry what it does is it contaminate it has allergen, so it contaminate the grains when you export to the you know, Asian country, they don't take that because they are allergic to that allergens in CRI. So the idea there is and it's very difficult there's no inseason chemical you can try and control in wheat unless you have herbicide resistant weed like waxy and wheat or Learfield weed where you can spray some of the herbicide and get rid of those grass species. So in those situation against this see destruction can really really help folks not letting those weeds eat grains in the in the crop grains.

     

    Is there a limit in the seed size? Or? I think that new technology sounds excellent for being able to destroy the seed in the field, or the limit that in terms of which species would be vulnerable.

     

    Yeah, yeah, those are all questions we are trying to address here as a future research in Australia, they have destroyed these rigid ryegrass that's quite a bigger size like a wheat grain size of the wheat seeds we are talking. But the things we are talking here like big weeds, very tiny small black color seed and waterhemp or Kosha. They're very tiny, tiny seeds, very small seed seed weeds. As per my experience. I have gotten the unit last Wolsey last fall September and we put together there was a technical team came and put on a combine and let's try that one of the grower field, we took it by miles south of Hayes and run on a grower farm was heavily infested with the Palmer Amaranth. I couldn't see even a sorghum plant, as all Palmer Amaranth. And I was trying to do that. The idea was how that goes, I was very curious how much destruction it can do especially in crop like sorghum, when it's green, and you know, high material, you're going through the combine what kind of destruction it can do, I was very, very curious. But somehow I found that we collected some of the samples out of the combine, and behind the Combine of that destructor I was always amazed to see like 85-90% of destruction is was doing on those Palmer Amaranth seed, those tiny, tiny seed was kind of pulverized. It was like powder form after that. So I was pretty amazed. So I was telling my team of folks from Iowa State and University of Arkansas, we're gonna run this in soybean, corn, as well as sorghum plots in the coming season to see if what it does and what how the crop species or the how the crop varieties also matters, using this technology, not only weed species, and then how the environment impact those results in high plane versus Midwest versus mid south, how things change from region to region, crop to crop, weeds to weeds. And with this, this grant, we have also a Ag Econ person on the team. So I'm going to look at the economic side of it. Because as I said, economy drives everything. And if you're gonna promote this technology, where we stand in terms of economy, is it cost effective? Is it sustainable? So I think I'm telling more future research here. But that's, that's going to happen.

     

    Good. Good. Sounds promising. Yeah.

     

    Pretty interesting, pretty exciting. And along with that, we are also not looking at one tool at a time. Our main mission with this project, which we got funded by NIFA, based on our TFS grant was to having bringing all the tools together, it's like bringing little hammers together. So we have a cover crops early in the season, we have herbicides applied. And then at the end of the season, we're gonna do see destruction versus Jeff lining, and comparing with what growers are normally doing conventional harvest. So there are three different approaches, we are trying to bring in one growing season, to say, hey, early season management with the cover crop, herbicides, late season management, or weed seed management, with this destructor or outlining how they come together as a system, and help growers if they're struggling with some of these multiple resistant pigweeds.

     

    I appreciate your mentioning the seed grant and appreciate you having come to Manhattan to present the results of that work recently. And that information will be up on our website in the near future. We'll have all of those and have those available for anyone to listen to, as well. I'm glad to hear that it panned out into a larger grant. So that's great.

     

    Yes. And that was really, really good support to get that kind of grant and reach out to the folks what they're really looking for the survey we did me and Sarah, we learn a lot. And some of that information. We just plug in our proposal. And it sold out pretty quickly. And to your surprise, and to my surprise, that proposal was ranked number one in CPPM in the country was in that program, NIFA CPPM program and the Secretary with agriculture wrote a letter to the PI. That was excellent proposal to put together for such kind of strategies to look in the soybean system.

     

    Congratulations on that. That's great. 

     

    Yeah, that's, yeah, that was really, really a great help from the TFs good Add money in that we could create some data to supplement data for the proposal.

     

    But you know, the phenomenon of resistance is just creeping through agriculture. So it's the herbicide resistant weeds. It's the fungicide resistant pathogens. It's the antibiotic resistant bacterial. And we really need to get a handle on it, if we're going to continue to produce at the levels we've been producing. So I'm wondering if the strategies you're looking at it, if there are some general principles that you think will be helpful in, in the other arenas, as well, not just the herbicide resistance, but in the others?

     

    Yeah, the basic principles, we are looking at the diversity in our system, I think, diversity is the key for sustainability, you keep doing one thing again, and again, you will see a problem that we have seen in our herbicide based methods of weed control, you've been doing same chemistries over and over, we have seen resistance issues, diversity, could be anything diverse cropping systems and diverse, you know, diverse methods of weed control, doing different things, you don't give same thing to that we don't do that best again and again, that that test start adapting to that matters or that strategy. So every year you change that strategies and give something new to the past and head those past with a different approach. So diversity, I think, is the key, what we are trying to achieve with this eating greater weed management system or ITM systems that you bring diversity in crop diversity in your herbicide diversity in your read species, overall system wide. I think that's the key principle we are looking at it. And that can be translated easily to the other disciplines, like, as you mentioned, plant pathology or entomology, not to look at one strategy or one thing at a time, but looking at the system level, where things can be bring and can bring that diversity into the system.

     

    I love this area. You mentioned that there are a lot of opportunities right now for weed scientists. And I look at the agronomy department here at K State. It's been really strong in terms of the scope of capabilities, the expertise that's in that department. It's pretty impressive what they've got within one department. So what if there are students that listen to this the either graduates or undergraduate students listening to this? What skill sets? Would you recommend chemistry? I mean, ecology, what skill sets would you recommend if they want to help tackle this problem?

     

    Yeah, that's a great question. As I mentioned, a lot of opportunities coming for fresh graduates and a lot of weed science positions recently opening up in academia, industry and other public sectors and private sectors. What I see as the weed scientists in this position, the four most important skill sets I can see is the knowledge of field based research, field based Weed Science Research, every fresh graduates they need. And then training of all the plants, science, biochemistry, physiology, genetics are those are specialized area already there. If you can take little bit of that have some expertise, you don't need to be doing five different projects in that area. But if you have little, little component of those areas, that really, really help understanding the problem, you know, from the root stand point of view, but applied Weed Science, statistical skills, how to handle the data, because the future is all about the data. With all this digital agriculture, you're going to tackle with the big data set, how to look at the data, there is a lot of data but what you make of out of the data. So statistical analysis, or analytical skills are also very, very important. And then you can also look at the mysteries in Weed Science, especially herbicide you need to know what you're doing and what you're tackling with. Because again, 70%, more than 70% of the calls the growers give me is they asked me the option herbicide option. They don't ask me, Hey, should I try this cover crop? They simply asked Hey, can I spray they can buy glyphosate is not working? How expensive? Is there a generic one? Is there a lower price one what is the formulation? All kinds of chemistry related question will come if you are going to go to those real world situations like applied weed sign, you know Precision Ag or engineering side of it. If you can learn some of the skills. I think that's the benefit as well, because that's happening right now. Preseason agriculture tools, a lot of weed science folks, they have started really using it and implementing into their programs. And that's going to be the future. A lot of the industry investment is going into that digital agriculture, especially from pest management, especially from weed management perspective. So those are some of the skills I just listed is applied Weed Science, applied field based research, chemistry knowledge, little bit of those physiology, genetics, biochemistry is knowledge, statistical analytical approaches. And procedure neck, I think, if you have little bit of all of those, and you can sell yourself, you will get the job, I'm sure. But for the weed scientists, as far as I know, yeah.

     

    Thank you. Thanks.

     

    Great question. And great, good bit of information for the students here on campus to file away as they think about what they want to work on. Yes.

     

    And I think I would also encourage undergraduate students if they are interested in in ag and if they are specifically interested in in weeds or any other pairs, they should do some project, they should contact folks on Main Campus or research center to get involved and to get learn how to handle the project or what to do in terms of research and how the research is conducted and how the data is handled. That's pretty basic. But there's quite a bit of learning before you get into your graduate schools, or Masters or PhD. If you can do a little project in undergrad that'd be really, really helpful.

     

    I enjoyed this conversation quite a great. One other big challenge on the horizon is, of course, climate change. And a number of studies done on how it's impacting the migration of plant populations and impacting fertility of some plant species, things like that it does that come into play here in terms of weed management?

     

    Yes, exactly. If you talk about climate change, or drastic changes in environmental conditions, weaves are one of those first pieces who will adapt to these changes, because they have highly diverse genetic background. And they have already been doing that molecular weight science program in Colorado State has been looking at Kosha from different angle. So they're trying to sequence the whole genome, they're trying to characterize some of the genes, good genes, they call it good genes, which are helping this Kosha to adapt cold treatments, or frost or drought, or heat, or salt, or even herbicide resistance, how those genes can be incorporated into our crops to make them more resilient for the future. Okay, so that's kind of angle to look at these weed species, we have that gene pool in those species, why don't we characterize and understand then how, and what they can do when we incorporate those gene in our crops for the future crops that can be resilient to the, to the these changes in climate environment. But as I said, changing climate changing environment, adaptation is going to be happen, evolution is going to happen in those weed species. Along with that, what's going to happen is interaction of the chemistry with the plant and the environment is going to change. And that's very critical to understand the efficacy of some of the chemicals we are seeing now probably will not be there into that future environmental future climate. Just because plant adapt, and they adapt differently, they have TIG cuticle, for example, the chemical may not penetrate that cuticle in the future, and cannot give you 90 95% control versus less than 70% control. So the efficacy is going to change or with increasing temperature or increasing carbon dioxide, C three C four species who's going to win and depending on those weeds species are those C three or C four, the shift will happen. And there'll be lot to play with climate and the principles of precipitation, how the precipitation change globally, some of these root shifts, also gonna share some some of the prediction has been done. Okay, if Great Plains start getting more rain, for example, we start going to see waterhemp coming this way, in Great Plains, if it's going to get more drier. Kosha is going to start going towards Midwest. There are predictions happening. And I think that's true, based on the biology of those weed species and based on the history of those species, how they have infested, and they have line ated themselves in those geography based on the climate. 

     

    Vipan, you had talked about when you were first over in the US you were working in Louisiana State working on cotton. And with climate change, I'm sure that that impacts this we're seeing cotton work its way into Kansas cropping Are you seeing? I mean, I know your focus is on the weed side of things. But are you seeing some of those other types of crops moving in more and more into these areas, some of the crops that we're used to moving Further north and having some new impacts of weed stress and that type of thing coming in with these new prompts.

     

    Definitely, with changing things with the changing environment and climate, these things are happening. And we need to be very resilient in terms of adopting those things, changing things like we were doing this faculty meeting other day and prioritizing our missions for the unit other days. So one of the priority we have have for next 1015 20 years is to look at alternative crops, new crops, basically what folks need, provided that our conditions get changed, our environment gets changed, we get less peace, we get more dry land, what are the alternative crops, things like barley, millet is number one can be adapted to in the West, that has not been expanded. There's a lot of potential for that crop. There's a lot of potential for canola in the southwest Kansas. That has been happening already happening expanding. In as you mentioned, cotton, yes, it has gone up. It was not the case five, six years ago, but it has gone up 300,000 acres of cotton in Kansas, can you imagine. And then over the top of that you can see the changes, the commodity Commission's have started funding some of the positions for those areas as well, they are looking for a pattern specialist in Kansas, they can support this. So things have been changing with the climate change with environmental change, as well as you know, other changes. And one thing I can I can say for sure, from a read science perspective, you bring new things, new crops, for example, that has long term impacts on our weed population. Some of the previous studies, long term studies, 1020 years long term studies have shown that the crop rotation in competitive crops and what kind of crop you're growing, will have ultimate impact on those wheat population. If you are growing, for example, let's say highly competitive crop like corn, or could be any cereal grains, that grows pretty aggressively, it can shift some of those wheat population over the time, a study done in Nebraska has shown that you keep doing this corn soybean rotation, you will see more and more issues weather resistant Kosha and resistant big weed, but you will bring cereal into the system, you will lower down some of those resistance issues is because the crop competition expressed those cycles of those weed species and don't let them produce seeds. So weight shift is going to happen when these crop change is going to come into play in our system. But as again, I said we have to be very resilient and proactive, like things are happening. And it's going to happen, especially from climate change standpoint. So we need to be resilient, or what alternative crops we can grow. And we can still make these folks or the growers more profitable in the future. Considering all these constraints, weeds and other pests we will have.

     

    Yeah, I'm hoping for mango and oranges.

     

    I'm not sure on that. One more. Yes, really,

     

    This has been a really a fun and interesting discussion. Well, thank you so much for your time. And thank you, Jim, for joining us as well. Do you have any final remarks, or any questions you might have for us before we sign off?

     

    Well, I would like to thank you both for your time. And also I like to reiterate that the support I got through the GFS Grant was pretty timely, and very supportive. And I could develop that project based on that information. So I would keep looking at future opportunities from GFS folks that I can come up with and collaborate with folks from other disciplines. And I would encourage young faculty at K State to look for those opportunities. And to come up with ideas there where they can collaborate with folks like me sitting in Hays versus in you know, in Manhattan and we come to know each other. That's a great opportunity and really appreciate all the support you guys have.

     

    So glad it worked out well. And thank you for your efforts. They're very much.

     

    Thank you.

     

    If you have any questions or comments you would like to share check out our website at https://www.k-state.edu/research/global-food/ and drop us an email.

    Our music was adapted from Dr. Wayne Goins’s album Chronicles of Carmela. Special thanks to him for providing that to us. Something to Chew On is produced by the Office of Research Development at Kansas State University. 

     

     

    The Many Paths of Pathogens with Dr. Philip Hardwidge, associate director of the Center on Emerging and Zoonotic Infectious Diseases

    The Many Paths of Pathogens with Dr. Philip Hardwidge, associate director of the Center on Emerging and Zoonotic Infectious Diseases

    In this episode, we host Dr. Philip Hardwidge, associate director of the Center on Emerging and Zoonotic Infectious Diseases in the Department of Diagnostic Medicine and Pathobiology at Kansas State University. Dr. Hardwidge’s research focuses on understanding, treating and preventing diarrheal disease caused by bacterial pathogens. These pathogens represent important threats to food safety, biosecurity and animal health. His research team is tackling the fundamentals of biochemical interactions, leading to a better understanding of mitigation methods.  

     

    Transcript:

    The Many Paths of Pathogens with Dr. Philip Hardwidge, associate director of the Center on Emerging and Zoonotic Infectious Diseases

    We have to be as scientists extremely open and and generally willing to share data be transparent about our
    raw data and like other aspects in life know when to ask for help.
    [Music]

    Something to chew on is a podcast devoted to the exploration and discussion of global food systems produced by the Office of Research Development at Kansas State University. I'm Maureen Olewnik. Coordinator of Global Food Systems.

    I'm Scott Tanona. I'm a philosopher of Science.

    We welcome back co-host Dr. Jim Stack Professor of Plant Pathology.
    Diarrheal disease caused by bacterial pathogens is a challenge in both humans and animals in many instances the introduction of pathogens in animal systems causes illness and in some cases is carried through meat processing affecting contamination of food meant for human consumption. Studies of food safety at K-State includes fundamental through applied research. The importance of research in the area of pathogenic bacteria has been addressed in several of our podcasts to date. Most focusing on the applied research in testing, monitoring, and mitigating potential contamination of food products. However, the basic molecular biology of host pathogen interaction is not well understood. In today's podcast, we will talk with Dr. Philip Hardwidge, Associate Director of the Center on Emerging and Zoonotic Infectious Diseases here at K-State. His study of host pathogen interaction has led to a better understanding of the mechanisms by which pathogens enter and colonize in a host system. With studies leading to an understanding of how this impacts autoimmune disorders, cancer, and more.

    I would like to welcome Dr. Philip Hardwidge to the podcast. Dr. Hardwidge is the Associate Director of NIH and Cobra Center on Emerging and Zoonotic Infectious Diseases. I am hopeful that he will explain to us exactly what all that means. Before we get started in talking about your current activities, Dr. Hardwidge could we maybe get a little bit of understanding of who you are, what your background is, and what brought you to K-State. What brought you to the area of study that you're in, today.

    Thanks for having me on this podcast series. I'm from the midwest, Michigan and Illinois. My father was a Pfizer scientist and we happened to be living in Central Illinois when I was a high school student, so he gave me some interest in Microbiology and Chemistry, so I ended up doing a Microbiology degree at the University of Illinois, and wanted to develop a research program kind of at the interface between Biochemistry and Microbiology, so I knew from a fairly early age where my career would hopefully head. I did a PHD at the Mayo Clinic Graduate School
    in Rochester, Minnesota. So, Mayo is a very famous hospital. They also have a very robust graduate training program. And after that, I did a postdoc at the University of British Columbia in Vancouver Canada. Primarily because one of the leading E Coli Microbiologists was running his laboratory in Vancouver and when I finished my education I took an Assistant Professor Position in South Dakota State University back in 2005. There were some unique opportunities to help develop their graduate program, and I had the opportunity to work with germ-free piglets which are a very nice model for some types of E Coli diseases.
    I was then recruited to the University of Kansas Medical Center and then subsequently recruited to Kansas State University where I've been since 2012.

    Great, thank you for the overview there. In the introduction, I referenced the Center on Emerging and Zoonotic Infectious Diseases. Can you tell us a little bit about what that is at K-State and what your goals are there?

    Sure, so we call this CEZID, Center on Emerging and Zoonotic Infectious Diseases. This is an NIH Center that's administered by Dr. Juergen Richt and myself. So, [Dr.] Juergen is a Virologist. I am a Bacteriologist and we're both interested in looking at virulence factors. So, namely what features of pathogens. What components of bacteria and viruses cause diseases in humans and animals. We're also looking at host pathogen interactions. And to do this we use basic science. So fundamental aspects of how bacteria and viruses work. How they cause disease, and we also take translational approaches that can be InVitro in test tube[s], lab experiments, or in large animal models of disease. So overall, we're attempting to advance our understanding of new or emerging infectious diseases, or zoonotic diseases, those pathogens that can cross the interface between animals and human beings. So Covid-19 is a great example of both cases. It has recently emerged, and it is Zoonotic. It's believed to have originated from bats, so you know within this center. [Dr.] Juergen and I administer the day-to-day operations, but we’re heavily interested in mentoring junior scientists. So, there are four primary projects each Principal Investigator works on a different pathogen that examines one emerging or zoonotic infectious disease and then there are five pilot projects. So smaller projects we funded with some seed money, and then there are two research cores, uh, to help develop the research infrastructure here at K-State.

    Sounds like it's a great center, and could you say something about why zoonotic diseases deserve such a focus, or what's important about them?

    Okay yeah, so obviously we've learned a lot more, or the public has learned a lot more, with the Covid- 19 pandemic. But you know zoonotic disease diseases, or zoonoses, are diseases caused by viruses or bacteria that can spread between humans and animals, or animals and humans. So, in many parts of the world there's very close association between animals and humans. So, farming systems can be quite different compared with what we're familiar with in the United States. So, there's very close contact between humans and animals and some good examples would include Influenza. So, we have the flu circulating in chickens and in pigs. These viruses can mutate and suddenly become able to infect human cells and cause disease. Antibiotic resistance is an issue. So, we've tended to treat food animal diseases. So bacterial infections of pigs and cows with very large quantities of antibiotics to control their infections and to promote weight gain. Well, this can evolve antimicrobial resistance in these organisms and some of these pathogens can also infect human beings. So, antimicrobial use in agriculture can have a direct impact on our ability to treat human infections. And then there are vector-borne diseases. So, bacteria and viruses that can be spread or transmitted by mosquitoes, ticks, and fleas. For example, so that the insect, the mosquito or flea, can provide a conduit between an animal and a human being. So, as the mode of transmission. So, many of these diseases are extremely serious. They're relatively new. They're emerging, and the the life cycle, this animal to human interface, explains why they're why they are called a zoonotic diseases.

    Great, thanks! And could you describe two, sort of, some of the different projects you, said there's a course of on going ones, and then some pilot ones. So, I don't know which, pick a couple just to give us a sense of some of the work that's going on in a little more detail. love to hear some more.

    Okay, so yeah, within our CEZID program, we have four very exciting primary research projects. One of them directed by Dr. Tom Platt, in the Division of Biology, looks at a pathogen known as shigella flexneri. Shigella has a lot of similarities to the hemorrhagic E Coli, the hamburger E Coli, E Coli o157 h7. He's very interested in the environmental behavior of Shigella. So what must Shigella do to survive in aquatic environments versus survive when Shigella has colonized a human host. And many of those molecular mechanisms are very different. The pathogen has to do, you know, many different things make many different proteins depending whether it's living in the environment, or within a human. [Dr.] Stephanie Shames studies Legionella. So, many of you may have heard
    the term Legionnaires Disease from this outbreak in the air conditioning vent in a convention in Philadelphia many decades ago. So, Legionella is the pathogen that causes this human disease. And [Dr.] Stephanie studies some of the specific proteins that legionella uses to subvert host defense mechanisms. Bacteria and viruses have evolved
    very elaborate mechanisms to short-circuit or subvert our natural host defense. My own laboratory studies those mechanisms as well. So, that's [Dr.] Stephanie's focus. We also have a Flavivirus project, Japanese Encephalitis Virus, Yellow Fever Virus these are Flaviviruses. [Dr.] Scott Huang is trying to design live attenuated vaccines to control flaviviruses. And finally, [Dr.] Nick Wallace is studying Papillomavirus. Papilloma Viruses are believed to cause non-melanoma skin cancer, but the mechanism is not understood. So, [Dr.] Nick's studies are designed to start to understand at a molecular level what's going on between Papillomavirus and skin cancer.
    If I could ask a question, you indicate that you're looking at certain proteins to maybe influence the outcome in a human infection, or an animal infection for that matter. We have a lot of vaccines for viral diseases, but relatively few for bacterial diseases. What's the strategy for, you know, using these proteins to prevent disease?

    Okay so, if I understand your question right. There are therapeutic strategies so we have ways to treat diseases that have already occurred, or we have preventative measures such as vaccines. Bacterial, many bacteria, are challenging to tackle with vaccines. There can be great variation in the surface of bacteria. The outer membrane proteins, for example, are often targeted by vaccines. This is essentially the outside of a bacterium. These can be very highly variable between strains among different strains. So, it's hard for a cocktail of vaccine proteins to really be effective in preventing disease. There's an emerging strategy known as anti-virulence compounds where we attempt to not kill the bacteria using antibiotics, but we attempt to subvert the bacterium's ability to cause disease. That's also something my laboratory is developing.

    How does that work?

    Okay, so the the classic antimicrobial penicillin, for example, you know, inhibits cell wall integrity in the bacterium, so the bacterium lyses and dies. There are many antibiotics used that block the ability of a bacterium to make new protein, bacterial cell then dies. Resistance is a major problem here. So, the more often you challenge a group of bacteria with these antimicrobials. The more frequently you select for mutants mutations in the bacterial genome that will allow it to resist those antibiotics. So, one emerging concept is to not try to kill the bacterium, but try to simply block its ability to colonize a human host, or secrete a toxin that would be deleterious to a human or an animal. And that's thought it would be much less prone to the evolution of resistance mechanisms. We're not trying to destroy or kill the bacterium. We're simply targeting a very small component of its biology, namely its ability to cause disease. Some of these targets are a little less obvious to identify. So the cell wall is a very obvious and effective target for antimicrobials. Some of the mechanisms that bacteria use to block the immune system. These are being targeted for these anti-virulence therapies, but they've only been identified and studied relatively recently.

    Yeah, thank you. So yeah, so that's cool. So the idea is not just that we haven't targeted this stuff, yet. So it's new, but that even ongoing application of these to it's basically blocking a side effect of what the bacterium's up to. Right? And lets it still propagate and continue on, because you're not sort of killing off only a subset of them. You're not forcing evolution, right? So, that the virulence isn't part of isn't something that it would sort of act against.

    Exactly, that's the concept. So, a good example is perhaps the hemorrhagic E Coli this is a devastating disease. If humans acquire this organism, but it's typically an accidental infection, so through undercooked ground beef, contaminated produce, humans occasionally get E Coli infections. Extremely serious, potentially fatal disease but this organism lives very naturally in the intestines of cattle. So, it's a commensal organism. It does no harm to the cattle. So, it's not really something that one would would do a blanket antimicrobial attack on. There there may be a mechanism by which we can have a more selective targeting of their virulence functions. So prevent disease but not select for uh resistant strains of this organism.

    Yeah, that's that's really neat, and and you said you're looking at some of those these interactions too in your own lab. Could you say what you're working on in this area?

    Yeah so, I became interested in this area through some serendipitous scientific conferences, a few years ago. My lab had always been interested in the biochemistry of bacterial proteins that are able to block the immune system of the human host. So, some bacteria such as E Coli and Salmonella have a secretion system. So, they have a nano scale needle and syringe like machine that allows them to inject proteins into the intestinal cells. They are colonizing so this nano machine allows the bacterium to manipulate the human cells, and try to prevent the cells from establishing a dialogue with the immune system. So the biochemistry of these proteins, how they've evolved to bind signaling hubs in this innate immune system, what enzymatic activities they have to inhibit host proteins. Very fascinating to me, but it became clear through some collaborative discussions, that some of these proteins might be good targets for anti-virulence therapies. And they also might be good model proteins that could be used to study the immune system more generally. In other words, bacteria and viruses have evolved to inhibit the immune system. Can we use some of their examples to build other drugs that would function as anti-inflammatories. That's kind of the direction my lab has gone in the last five or six years.

    So, you're turning this then to not just how can we prevent more more disease, right sort of, how can we learn from from what these bugs are doing to actually address other concerns like right?

    Exactly, so, if we take, and that's you know that's the value of of basic science and basic molecular microbiological studies, if we really take a close look at what nature has already done. What evolutionary pressures have selected for, we can learn a lot about how organisms interact with each other. And specifically with regard to the immune system and inflammation, we can see very clear examples from bacteria and viruses. They have very very effective anti-inflammatory strategies. So E Coli and Salmonella for example, are great masters of inhibiting inflammatory responses. Well, if one looks at other diseases, other human diseases, such as Psoriasis, which is skin inflammation, cancer, diabetes, inflammatory bowel disease. These all have some common features in that some of the inflammatory signals proteins known as Cytokines are overproduced in too high abundance. So, can we take some of the bacterial strategies that block the production of these proinflammatory proteins, take the bacterial proteins, modify them detoxify them make them friendly for use, and turn them into lead compounds for new new drugs. So that's an emerging area of many laboratories. It's a concept known as drugs from bugs. The bugs are the bacteria and viruses perhaps. They can suggest to us novel therapeutic strategies to controlling inflammation.

    Nature did it first.

    Nature did it first. So, let's learn from nature. There are many ways to, you know, look for potential new therapeutics. There's random libraries of small molecules, there are you know very robust computational strategies, so letting machine learning, computer strategies to predict chemical interactions, or take a look at what bacteria and viruses have already done.

    The work is fascinating and clearly taking a lead, from as you said, what nature has already done is obviously a very effective approach. I'm wondering, with the new N-bath Center going in, kind of in your backyard, what kind of interaction do you see going on between the work you're doing and that center? Is it going to be, kind of running parallel to one another? Do you have direct input in what's happening up there, or direct activities going on in the future?

    So, there are obvious parallels between the, you know, the National Bio and Agro Defense Facility, NBAF. It is literally right next door to our CEZID program, and to our laboratories. So, we're very interested in looking for partnerships certainly there will be a lot of training opportunities. So much of the workforce at NBAF is likely to come out of K-State. So, one of our missions is to train this new workforce, both with the book knowledge and the hands-on laboratory skills that will make them good contributors to NBAF. There's a lot of parallels in the mission, you know, so to protect the food supply, to protect agriculture, to protect the population against zoonotic diseases. So, there's good interface between CEZID and NBAF. So, definitely we're interested in establishing collaborations. Dr. Juergen Richt, my collaborator, he's already established several collaborations. We work with the Plum Island facility, already. This is the laboratory that's essentially moving from the Long Island area to become NBAF. So many of the projects are already in place, and certainly we'll see a lot of growth in the coming years about how to safeguard food animal health public health and really preserve our agricultural economy from various threats.

    Earlier, you mentioned the E Coli outbreaks that have occurred as a consequence of produce and because of those outbreaks over the past, probably five to ten years, been a lot of research looking at E Coli and Salmonella in particular, and whether plants actually play a role in their life history. And clearly they they do. That it's not really just an incidental occurrence of those organisms perhaps being sprayed on these these plants with irrigation water or something like. That in fact there's compelling data that they truly infect the plants and that they've actually documented an upregulation in expression of effector genes, and things like that. So, there seems to be some strong relationship between E Coli Salmonella and the plants. And I'm just wondering if you're aware of any evidence of that plant component actually helping to drive the evolution of those species or the emergence perhaps of new pathotypes. Because we we know that the plant pathogenic bacteria as well as as some of these share secretion systems and that some of our closely some of the plant pathogens that are closely related to the enterics, you know, have multiple secretion systems. And I'm just wondering if there's any evidence that you're aware of that there's a drive in the evolutionary process for some of the zoonotics? Some of these human pathogens like E Coli or Salmonella.


    Yeah, that's a fascinating question. So thanks for bringing that up. It's very clear that a lot of what bacteria do when they interact with human or animal cells also occurs when bacteria need to interact with plant surfaces. So for example, the elaboration of surface appendages for adherence. The pili, the the swimming apparatus called the flagellum, these are all up regulated by by contact with plant surface. Gaining entrance at wound sites or cracks between root hairs there's a lot of physiological similarity between the human intestine and some aspects of plant cell boundaries where these mechanisms are conserved. Whether plant interaction drives the evolution of bacterial pathotypes, or not, is unfortunately rather poorly understood. And I think a lot of that is a function of scientists taking a human-centric view to a lot of what these pathogens do. So, we tend to take the view, and I'm also to blame, bacteria exists to cause disease in people to cause diarrhea, in terms of these intestinal pathogens to cause respiratory illness, of course that's not the case. So, most infections are accidental. The only goal, if a bacterium were to have a goal, is to replicate so to find food source and to replicate. So, our view is often a little bit warped, and we don't give appropriate coverage to what might be going on in the environment. So when new virulence factors emerge. Are sporadic accidental human infections really driving that evolution? Most likely not. More likely the interaction in the between the bacterium and its environment, whether that's in water or with produce, on spinach or lettuce, this is more likely the the driving factor to evolving new bacterial path types. But it's poorly understood. Some of the model systems are are less well developed. Plants also have immune systems, so I've mentioned a few times about an ability of these bacteria to inhibit human immune systems. Well, plants also have immune responses to infection. They essentially try to wall off the infected cells to limit the spread of the infecting pathogen. But again, these bacteria have ways to evade that plant immune system. Secretion Systems are well conserved in how they block human versus plant functions. So, I think this area should be studied much more extensively. I think if more funding were available to really look at the what forces drive the evolution of these bacteria we would learn much more. To some extent, that's a product of our funding system. In that it tends to be slanted towards immediate tangible therapeutic benefits to humans or food animals, and at times basic science inquiry can be ignored or at least underfunded. So, I would target as you suggest this area as a potential rich area of investigation.


    I was just going to say your center must be a rich experience for the students. I'm just wondering, how do you maximize that? I mean with the aggregation of expertise you have there and the diversity of projects that could be a pretty rich environment for certainly a graduate experience. I was just wondering how you might comment on it?

    Yeah, thank you. We're very excited we're in the second year of this program and one of the main goals is to bring along the next generation of of scientists. So, not only are we mentoring the the junior faculty, you've heard some of their research projects already. Another component of that is mentoring their own students, and postdocs students, can be undergraduates, masters students, PHD students, and the mentoring can be direct or indirect. So, certainly we've seen pretty significant growth in student numbers. Each lab seems to be getting more and more students. We've started several journal clubs. We've discussion programs. We bring in distinguished scientists to speak with students and faculty. So, we try to bring in leaders of the of the various fields have scientific discussions with students. Really set the foundations for their growth, as we've discussed, we do need an immediate workforce as NBAF comes online. But, we also need the the next generation of basic scientists to populate our university laboratories. So, we're also seeing a rapid increase in research infrastructure. So, several new pieces of technology are now new to K-State. So we have single cell capability. So, we can isolate single cells from various tissues work with them in isolation do single cell sequencing, single cell gene expression, analysis. We have new live cell microscopes again. This was not available here until recently. So, I'm very excited that we have a really first-rate group of faculty. We have many people interested in highly capable of student mentoring. And we're really developing first-rate technology, so students can get hands-on experience with techniques that will make them very marketable to academic or industry careers.

    In many respects progress is a function of the relationship between science and technology, and probably for the last 500 years it's been kind of a push-pull relationship where one feeds the innovation of the other, or feeds off of the innovation of the other, and it is more of philosophical question. Do you think at this point though we science has become more dependent upon technology to the point where it influences the questions that we ask?

    I think there can be a tendency for that. So it's very easy to get excited about a new machine, and then frame scientific questions around what that machine is capable of doing. So for example, if you get a new live cell microscope the tendency can be, let's do all experiments focused on live cell microscopy. But, I'm not too concerned. I think you know good science is done through the classic scientific method, and tried and true technologies are often still the best approach to solving problems. So for example, my postdoc yesterday showed me a very nice set of data that have solved a year-long problem for us. He was using genetic techniques in Salmonella established in the 1960s. But he was he was aware of the older literature, he could recognize the value of that more dated technology, and he knew it was appropriate for the question he was asking. So, it is a challenge, technology makes it some things easier. But, they can kind of cloud our understanding of some of the basic concepts. So, I think there's room for both, but I guess I'll point out that there's been a lot of very important scientific discoveries that are essentially accidental. They're serendipitous, so really it's our job to do well-controlled experiments, have testable hypotheses, have good robust record-keeping practices, and then keep our eyes open, because if we see something unexpected or unusual we want to be able to follow that up, and be confident it's not just a laboratory mistake. So, all the fundamental training students learn in Chemistry 101, in undergraduate, keep a good notebook, rigor, and reproducibility, I think you know these fundamental issues are always going to trump any technological advance, in terms of how we move this field forward.

    You've been talking about basic science a lot and about the accidental things that pop up right from just doing some work. And then, you know, as you said in your own work, you know moving to how this might, some discoveries, might actually apply to treating autoimmune disorders and things like that. Right? So, how do you think about either, you know, an individual scientist or the scientific community doing this balance of the, you know, let's work on some really basic questions that we, and we just want the answers to, and we don't really know what the application will be. To then, noting where there might be, you know, some something really useful applied and sort of starting to follow down that path. To then, sort of hey we have a very specific question. Like, you know, Covid, and we have to address.So how do you balance that as either an individual, or as a research group, or as a wider community? What thoughts [do] you have on that?

    Thanks, that's one of the toughest aspects, at least personally for me as a professor. How focused and narrow do we get in terms of the minutia of a protein. So, do we need to understand every atom of every protein? Versus when are we ready to apply this to a disease? Translate this into a real therapeutic. I think we have to be as scientists extremely open, and generally willing to to share data, be transparent about our raw data, and like other aspects in life, know when to ask for help. So, most of my translational endeavors in terms of anti-virulence compounds bacterial proteins used to inhibit the immune system have really resulted because I shared data very early on with with someone at a meeting, or a conference, or invited lecturer, and they had a slightly different way of of looking at the data. So, I viewed it in one direction. That I understand how this protein works, and an outsider to my specific field was able to ask a broader question. So, oh if you know that this protein blocks the immune system, have you ever thought about trying this? Or, I know a friend at my institution who could take your protein and try it in their mouse model of inflammation, for example. So it is a challenge. We're constricted by funding opportunities, so it's more comfortable to kind of stay siloed, and work on what we’re recognized as experts to do. But, I think it's far more important to take these leaps of faith, and try to work with translational scientists who might really see the application of your basic discovery, and be able to help you apply it to a broader application. And of course, there's a lot of complexities with university licensing and patenting data ownership, but we have all the university resources to help us with that.

    You've been talking a lot about individual organisms that you work with. Have you done any work with the way those organisms interact with one another different types? And what I'm kind of getting at is the microbiome activities that are being tested and looked at in various areas across campus. Does your work specifically get into that area?

    Yeah, thank you. We have worked a little bit with the microbiome, and that's something I haven't mentioned yet in this discussion. So, you mentioned the naturally occurring commensal, or beneficial organisms that that colonize all of our mucosal surfaces. So, the microbiota or microbiome, another aspect of the success of a bacterium or virus, if we think about a human infection it's not just the immune system of the host that challenges the pathogen. It's also the bacteria that line the respiratory tract, the intestinal tract, the genital tract, the skin. So, that's another barrier, and it's actually one of our defense mechanisms, essentially. So, how do bacterial pathogens interact with the microbiome? We've done a few studies in that area. So, we've mostly used mouse models to study where we can do fecal transplantation, to study what makes certain strains of mice more resistant, or more susceptible to bacterial infection. And a lot of that susceptibility is driven, not by the strain of mouse per se, but rather which intestinal microbes are harbored within that mouse. So, the microbiome really does play an important role in dictating disease susceptibility. So again, it's interesting to do a survey, to understand what bacteria are present in the gut, what are the correlates of resistance, and susceptibility. But, it's even more interesting, to then use that information to start to tailor different treatments, so the pre and probiotic industry is extremely robust in this area. There's opportunities for collaboration to make animals more resistant to infections, or are more robust at gaining weight over their development time. And there's also fundamental aspects of how the microbiome dictates immune responses so we may again learn a lot from how the microbiome can dampen the immune system in terms of generating new anti-inflammatory compounds. If you take an animal and you rid it of the microbiome, or you develop a germ-free animal in the laboratory, for example, you tend to see very potent anomalously high inflammatory responses. Suggesting, that one job of the microbiome is to dampen the immune system and really make it selective towards pathogens, rather than, towards random insults or challenges. So, this is a huge area to study. We've collaborated with several groups here on various organisms to do surveys of the microbiome, and we're hoping to apply some of this knowledge to potentially discover new therapeutic strategy.

    Fascinating stuff!


    Very much so, absolutely. Do you have any questions for us, [Dr.] Phillip?

    I think we've covered kind of the highlights, and I think I've given you a good flavor of how I approach science, and what my main interests are.

    Thank you very much. I enjoyed the discussion. Yeah, really enjoyed the discussion.

    Thank you, Thank you all.

    Thank you, [Dr.] Maureen

    Yeah, thanks much! bye-bye bye-bye!

    If you have any questions or comments you would like to share check out our website athttps://www.k-state.edu/research/global-food/ and drop us an email.
    Our music was adapted from Dr. Wayne Goins’s album Chronicles of Carmela. Special thanks to him for providing that to us. Something to Chew On is produced by the Office of Research Development at Kansas State University.

    Keywords: Center on Emerging and Zoonotic Infectious Diseases, Disease, Global Food Systems, Kansas State University, Pathobiology, Research