intravitalmicroscopy

Myosins team up to help secretory granules integrate

Actomyosin contractility drives a variety of membrane remodeling events, including the integration of secretory granules into the apical plasma membrane after exocytosis. By visualizing granule integration in the salivary glands of live mice, Milberg et al. reveal that myosin IIA and myosin IIB act at different stages of the process and that the activation and assembly of these myosin isoforms into contractile filaments is regulated by the F-actin scaffold, which assembles on secretory granules and recruits myosin light chain kinase. This biosights episode presents the paper by Milberg et al. from the July 3rd, 2017, issue of The Journal of Cell Biology and includes an interview with the paper's senior author, Roberto Weigert (National Institutes of Health, Bethesda, MD). Produced by Caitlin Sedwick and Ben Short. See the associated paper in JCB for details on the funding provided to support this original research.

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A framework for understanding muscle microtubules

The microtubules of skeletal muscle fibers are arranged into an orthogonal grid, but how this network is formed is unknown. Oddoux et al. reveal that the network is built by dynamic microtubules nucleated from Golgi elements. This biosights episode presents the paper by Oddoux et al. from the October 28, 2013, issue of The Journal of Cell Biology and includes an interview with senior author Evelyn Ralston (NIH, Bethesda, MD). Produced by Caitlin Sedwick and Ben Short. See the associated paper in JCB for details on the funding provided to support this original research.

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