mitosis

Broken chromosomes stay on the safe side in mitosis

Unrepaired DNA double strand breaks can generate chromosome fragments that lack centromeres but, surprisingly, these acentric chromosomes can nevertheless segregate to spindle poles during mitosis. Karg et al. reveal that, in Drosophila melanogaster neuroblasts, acentric chromosomes segregate along interpolar microtubules at the spindle periphery that are organized by the chromokinesin motor protein Klp3a. This biosights episode presents the paper by Karg et al. from the June 5th, 2017, issue of The Journal of Cell Biology and includes an interview with the paper's senior author, William Sullivan (University of California, Santa Cruz). Produced by Caitlin Sedwick and Ben Short. See the associated paper in JCB for details on the funding provided to support this original research.

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Pushing the envelope on spindle assembly

During mitosis, numerous proteins accumulate around the mitotic spindle to help it assemble and segregate sister chromatids correctly. Schweizer et al. reveal that a membranous spindle envelope facilitates the accumulation of these proteins by excluding large organelles from the spindle region. This biosights episode presents the paper by Schweizer et al. from the August 31st, 2015, issue of The Journal of Cell Biology and includes an interview with the paper's senior author, Helder Maiato (University of Porto, Porto, Portugal). Produced by Caitlin Sedwick and Ben Short. See the associated paper in JCB for details on the funding provided to support this original research.

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Dynein helps centrioles stick together

Dynein has numerous functions in mitosis, but the function of the motor complex's light intermediate chains is poorly understood. Jones et al. reveal that dynein's light intermediate chains are required to maintain centrosome integrity during mitosis, preventing the premature separation of mother-daughter centrioles and the formation of multipolar spindles. This biosights episode presents the paper by Jones et al. from the November 24, 2014, issue of The Journal of Cell Biology and includes an interview with the paper's senior authors, Viki Allan and Sarah Woolner (University of Manchester, UK). Produced by Caitlin Sedwick and Ben Short. See the associated paper in JCB for details on the funding provided to support this original research.

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Rained in on a paradise of a tropical island in French Polynesia..

Not complaining as things could be a LOT worse!!

Yet once the board games were exhausted, emails read and answered and even slotted a gym session, the urge to create something took over.

Hence uploading a little one, which appeared to have soothed the rather gloomy family mood..

Once back home, I will very shortly upload about 6 Deep House sets, the live NYD one and a set I really would like to offer to the community at large called "God is a DJ"..

But considering the 6 hours it took to upload this one thanks to neanderthalian broadband speeds here, here is a small "amuse-gueule" straight out of the mixer.

Sexy, Soulful with a sprinkle of deep progressive beats with one common thread big BIG basslines.

Playlist:

1. 2013 Dying to Survive (Original Mix). Freeman & Farrelly


2. 2013 Guess (Dj Lazz Mystery in The Deep). Deemah and Soledrifter


3. 2008 Sent From Above (Scott Wozniak Remix). Stephen Rigmaiden feat. Darryl D'Bonneau


4. 2013 If You Take My Love (Sound Blast Stereo Vocal Mix). Kings Of Soul


5. 2013 Sender (Original Mix). Paco


6. 2013 The Pressure (Shakedown vs. Layabouts Remix). Haji & Emanuel feat. Beverley Knight & Bryan Chambers


7. 2013 All About That Love (Original Mix). Roland Clark


8. 2012 Deep Down The Underground (Original Mix) Mike Dunn and Fabian Flores


9. 2009 Balearica (U-Ness & JedSet Dubtastic). Jay Vegas


10. 2013 Sexy Scissor (Petros Ecstasy Remix)
    


11. 2012 Prove Your Love (original Mix ?).  Petros Mitolis


12. 2013 Taste This Love (Petros Mitolis Hot Light Mix vs. Original Mix). Petros Mitolis, Val Gee


13. 2008 All This Love For You (Rocco Spoken Mix). Ralf Gum feat. Diamondancer

Highlights:

"Dying to Survive" I love Sade, she has one of the best catalogue of her generation. Unique original creations, amazing deep voice and lyrics that actually mean something. We are blessed to have her. As to this remix, I have been wanting to open a set with it for a long time . The bass line, the drums add a tragic dimension to a track that was already perfection..

"Guess" one of those little gems, by far not as simple as the synth hook would make you feel. If you take time to hear the rolling bass line it is pure genius and sustain the melodic throughout the song.

"Sent from Above' Scott Wozniak take on the old soulful standard. Scott showing one more time that he is one of the best ( if not the best) keyboard player in house music. He always adds an edge depth and weight to a track through his keys, . Impeccable production! Scott just crowd sourced the production of his first artist album, I can't wait for the result.

"If You Take My Love" from Kings of Soul.. By that time the set had taken a more heavy turn than anticipated and this track came as the obvious drop. Another BIG BIG bassline, this is sexy soulful house as I would LOVE to hear in a club and I ( modestly) think the paring with Sender was an inspired fluke.

"Sender" from Paco.. whatever this is.. Progressive, Deep, Indie.. I do not care! it is SO GOOD! Great production, great wide atmosphere..

Double dose of "The Pressure" with Beverley Knight as the ever amazing vocalist.. Progressive, sexy, soulful.. I guess I needed "release" as well after looking at the never ending tropical rain for 6 hours :)

"All About That Love" I love Roland Clark, and will always do. Most of you know that already. It was time in the set to go a bit deeper and once agian Roland served it up. Because " it is all about that rhythm, all about that love"..

"Deep Down the Underground" there are a few deep tech house tracks I love and this is the perfect example of what someone like Mike Dunn can do.. creating new sound, creating new beats.. Long life to those artists!

By now I felt like bringing a bit of sugar and "Balearica" was the obvious tune to drop.. I thought of truncating it as I knew I was about to run over the CD format but I doubt I will have to cut any CD of this one therefore here it is as a whole track with the amazing acoustic guitar in the second part of the track. Great percussion, perfect production.

Which takes us to Petros Mitolis little medley..

Petros Mitolis. greek german deep and sexy house producer.. I will never do him justince but please listen to his tracks and purchase them, deep, sexy soulful, wide, with imppecable production.. The best way I can describe his sounds... The perfection of Germany with the style and sexyness of Greece.

"Prove this Love" and "Taste This Love", if you like deep, sexy House buy both releases, there is a lot more there than I can showcase.

"All This Love For You" one of my base track when I want to go back to the roots of house and close a set.. Simple architecture, spoken words, which tell better than I ever would all the love I have for House Music..

Back as soon as I am on a faster internet speed.

Happy New Year!!!!

Xtian

The position of the mitotic spindle is controlled by the microtubule-based motor dynein and a ternary complex of the proteins NuMA, LGN, and Ga. Kotak et al. demonstrate that the ternary complex's primary function is to localize dynein to the cell cortex and that cortical dynein is sufficient to position the mitotic spindle in HeLa cells. This biosights episode presents the paper by Kotak et al. from the October 1, 2012, issue of The Journal of Cell Biology and includes an interview with author Pierre Gonczy (EPFL, Lausanne, Switzerland). Produced by Caitlin Sedwick and Ben Short. See the associated paper in JCB for details on the funding provided to support this original research.

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In early Drosophila embryos, nuclei undergo rapid, synchronous divisions without being separated into individual cells by cytokinesis. Telley et al. develop a cell-free assay to reveal that microtubule asters help disperse the nuclei throughout the embryonic cytoplasm, moving them to the right position for development to continue. This biosights episode presents the paper by Telley et al. from the June 25, 2012, issue of The Journal of Cell Biology and includes an interview with lead author Ivo Telley (EMBL, Heidelberg, Germany). Produced by Caitlin Sedwick and Ben Short. See the associated paper in JCB for details on the funding provided to support this original research.

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The ERM protein Moesin helps mitotic cells undergo a series of dramatic shape changes by linking the actin cytoskeleton to the plasma membrane. Roubinet et al. describe how Moesin's localization and activity is regulated over the course of the cell cycle by two different pathways that control Moesin phosphorylation and phosphoinositide synthesis. This biosights episode presents the paper by Roubinet et al. from the October 3, 2011, issue of The Journal of Cell Biology, and includes an interview with senior author Sébastien Carréno (University of Montreal, Canada). Produced by Caitlin Sedwick and Ben Short.

At the start of mitosis, the nuclear envelope and nuclear pore complexes break down and disperse into the endoplasmic reticulum and cytoplasm. Lu et al. use rapid, 3D live cell imaging to reveal that, in contrast to previous models, the nuclear envelope reforms directly from ER cisternae after mitosis and that this happens before nuclear pore complexes start to reassemble. This biosights episode presents the paper by Lu et al. from the August 8, 2011, issue of the Journal of Cell Biology, and includes an interview with authors Lei Lu (Nanyang Technological University, Singapore) and Tomas Kirchhausen (Harvard Medical School, Boston, MA). Produced by Caitlin Sedwick and Ben Short.

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Cells drastically alter their structure at the onset of mitosis. Gavet and Pines reveal that the mitotic kinase CyclinB1-Cdk1 triggers its own nuclear import in prophase to ensure reorganization of the nucleus and cytoplasm is synchronized. This biosights episode presents the paper by Gavet and Pines from the April 19, 2010 issue of The Journal of Cell Biology, and includes an interview with senior author Jonathon Pines. Produced by Justin Paul and Ben Short.

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A microtubule-independent network of proteins called the spindle matrix is involved in assembling mitotic and meiotic spindles, but whether the matrix makes a mechanical contribution to spindle shape is unclear. Gatlin et al. manipulate spindles with microneedles to directly probe the mechanical properties of the spindle matrix. This biosights episode presents the paper by Gatlin et al. from the February 22, 2010 issue of the Journal of Cell Biology, and includes an interview with lead author Jay Gatlin. Produced by Eun Choi and Ben Short.

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