antibody

Lynda Coughlan joins the weekly virtual bus companions for a discussion of a host defense peptide from frogs that destroys influenza virus, and mouse models for acute and chronic hepacivirus infection.

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler

Guest: Lynda Coughlan

Become a patron of TWiV!

Links for this episode

• Linda on Virus Watch and BSGCT
• Anti-influenza peptide from frogs (Immunity)
• Magainins (Wikipedia)
• Urumi
• Kathy's defensin paper (PLoS Path)
• World Hepatitis Day
• Mouse model for hepacivirus infection (Science)
• Letters read on TWiV 452

Weekly Science Picks

Kathy - US rivers as subway map
Lynda - No Time to Lose: A Life in Pursuit of Deadly Viruses by Peter Piot
Alan - Apollo-era computers found in dead engineer’s basement
Vincent - 110 NFL Brains, research article in JAMA and The best science is often accidental

Listener Pick

Stig - Security Now! and The Gene by Siddhartha Mukherjee
Ricardo - John Oliver on Vaccines

Intro music is by Ronald Jenkees.

Send your virology questions and comments to twiv@microbe.tv

Guests: Sharon Isern and Scott Michael

Sharon and Scott join the TWiV team to talk about their work on dengue antibody-dependent enhancement of Zika virus infection, and identifying the virus in mosquitoes from Miami.

Become a patron of TWiV!

Links for this episode

• Live at Florida Gulf Coast University (TWiV #111)
• Florida DOH Daily Zika Update
• Regional Zika update, Americas (PAHO, WHO)
• NJ Zika Bill
• Dengue virus antibodies enhance Zika virus infection (bioRxiv)
• First detection of Zika virus in Brazilian A. aegyptii (Mem Inst Oswaldo Cruz)
• 2017 ASM Scientific Writing and Publishing Online Course
• Letters read on TWiV 410

This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completely free if you sign up at curiositystream.com/microbe and use the promo code MICROBE.

This episode is also brought to you by Drobo, a family of safe, expandable, yet simple to use storage arrays. Drobos are designed to protect your important data forever. Visit www.drobo.com to learn more. Listeners can save $100 on a Drobo system at drobostore.com by using the discount code Microbe100.

Weekly Science Picks

Sharon - Zika virus comics and cartoons and Florida weekly arbovirus reports
Scott - Real-time tracking of Zika virus evolution
Alan - Evolution of antibiotic resistance on a mega plate
Rich -  Windytv
Kathy - Zika virus map and timeline
Vincent - Ohsumi Nobel advanced information and HR 5325 funding breakdown

Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Rich Condit

The latest Zika virus news from the ConTWiVstadors, including a case of female to male transmission, risk of infection at the 2016 summer Olympics, a DNA vaccine, antibody-dependent enhancement by dengue antibodies, and sites of replication in the placenta.

Links for this episode

• Zika virus disease in the US (CDC)
• Female to male Zika virus transmission (CDC)
• Zika virus in female genital tract (Lancet)
• Zika virus transmission in Utah (CDC and NYTimes)
• Olympic Zika virus risk (CDC)
• 15 companies on Zika virus vaccines (WSJ)
• Zika virus DNA vaccine in mice (Nature)
• Zika virus disease in Colombia (NEJM)
• Dengue virus antibody dependent enhancement of Zika virus (PNAS)
• Zika virus antibodies enhance dengue virus disease (Science)
• Zika virus in human placenta and developing brain (bioRxiv)
• Zika virus targets different human placenta cells (Cell Host Microbe)
• Letters read on TWiV 399

This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE.

Become a patron of TWiV.

Weekly Science Picks

Alan - CDC postmortem on Ebolavirus outbreak
Rich - Refutations to anti-vaccine memes (Twitter, Facebook)
Dickson - History of urbanization
Vincent - How to cut subject from background in Photoshop

Listener Picks

Marion - Skeptics Guide to the Universe podcast
Jennie - Leatherback turtles in Costa Rica

Send your virology questions and comments to twiv@microbe.tv

Host: Vincent Racaniello

Guests: Michael Diamond, Michaela Gack, Laura Kramer, and Charles Rice

Four virologists discuss our current understanding of Zika virus biology, pathogenesis, transmission, and prevention, in this special live episode recorded at the American Society for Microbiology in Washington, DC.

[powerpress url="http://traffic.libsyn.com/twiv/TWiV392.mp3"]

Click arrow to play
Download TWiV 392 (62 MB .mp3, 85 min)
Subscribe (free): iTunes, RSS, email, Google Play Music

Video of this episode at YouTube

This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE.

Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler

Preprint servers, the structure of an antibody bound to Zika virus, blocking Zika virus replication in mosquitoes with Wolbachia, and killing carp in Australia with a herpesvirus are topics of this episode hosted by Vincent, Dickson, Alan, and Kathy.

Links for this episode

• biorXiv preprint server
• Please review Vaccines and Your Child
• Structure of antibody bound to Zika virus E protein (Cell Host Microbe)
• Wolbachia blocks Zika virus in mosquitoes (Cell Host Microbe)
• Zimmer on Wolbachia and Zika virus (NY Times)
• Fauci on Zika virus vaccine (Science)
• Carpageddon (The Conversation)
• Letters read on TWiV 388

This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE.

Also brought to you by ASV 2016

Weekly Science Picks

Dickson - EarthEnv
Alan - Research funding by lottery
Kathy - Eugenia Cheng Math and Baking
Vincent - Zika Diaries

Listener Picks

Stephen - Virus trading cards
William - Virus trading cards
Norma+Maurice - Virus trading cards
Tom - Virus trading cards

Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler

Guest: Nina Martin

Nina Martin joins the TWiV team to talk about the movie Vaxxed, her bout with dengue fever, and the latest research on Zika virus.

Links for this episode

• Public Health United
• Fifty Shades of Science (TEDx)
• Parents convicted in death of toddler (Science Based Medicine)
• CDC: Zika virus causes microcephaly (NEJM)
• Dengue virus antibodies enhance Zika virus infection (biorXiv)
• Zika virus in Brazilian nonhuman primates (biorXiv)
• Zika virus IgM in CSF of microcephalic babies (Lancet)
• Brain organoids for modeling Zika virus infection (Cell)
• Letters read on TWiV 387

This episode is sponsored by CuriosityStream. Get two months free when you sign up at curiositystream.com/microbe and use the promo code MICROBE.

Also brought to you by ASV 2016

Weekly Science Picks

Nina - Vaccines and Your Child by Paul Offit and Charlotte Moser
Dickson - The animals of Chernobyl
Alan - Five rules of lab safety
Kathy - 20 best science images of the year?
Vincent - Massive undersea crab swarm

Listener Picks

JP - Global map of wind
Todd - Doc Martin

Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, and Daniel Griffin

Guest: Michael Libman

Michael returns to help the TWiP trio solve the case of the Delusional African Expatriate, and discuss the association of natural and induced antibodies in mice with differential susceptibility to secondary cystic echinococcosis.

Links for this episode:

• T. brucei life cycle (pdf)
• Natural and induced antibodies in echinococcosis (Immunobiol)
• The collaborative cross
• Letters read on TWiP 104

 Case study for TWiP 104

This week's case involves a young man in his thirties, admitted in hospital in Anchorage AK in early June. Chief complaint, severe muscle pain and tenderness. Usually healthy guy, 1 week ago had bad case of diarrhea with belly pain and vomiting, lasted a full week. Now has fever. Concerned because wife now is having diarrhea. Unremarkable history, unknown family history. HIV negative. All childhood vaccinations. Does not eat raw meat. No meds, does seasonal work, social drinker. Came back from successful hunt, got a black bear. Dressed in field, cooked at home really well. Wife also ate bear meat. Bear meat is in freezer. No vegetables. Also eats salmon which he caught the previous season, then frozen. Drinks water from the stream when he hunts. Physical exam: hot, 38.5, bp 115/75, pulse 105, breathing comfortably. Anxious, swelling around both eyes. Sclera not noted. Labs: WBC elevated 14,000, 30% eosinophils; chemistries fine; muscle enzymes LDH, CK elevated.

Send your diagnosis to twip@microbe.tv

Send your questions and comments to twip@microbe.tv

The paratenic hosts reveal a case of scabies in the Traveling Salesman, and discuss a role for natural antibody in protection from infection with Plasmodium.

Hosts: Vincent Racaniello, Dickson Despommier, and Daniel Griffin

Links for this episode:

• Parasite Nobel Prizes 2015
• Ivermectin story (Nat Rev Micro)
• Ivermectin target (Brain Res)
• Gut microbiota and Plasmodium protection (Cell)
• Natural antibody (virology blog)
• Image credit
• Letters read on TWiP 97

Case study for TWiP 97

Daniel has two cases this week. Two people with similar presentations. A 12 yo girl in Peru presented for care with painless dark brown area on right knee. Has brothers and sisters, often play together. No trauma, but area is expanding. Months later develops cognitive changes. Second case is a 5 yo boy from the coastal region of Peru brought in by Mother, has red swollen area on face. Mom says while playing scratched nose, swelling right cheek, eye, nasal obstruction, no response to antibiotics. MRI of both show infiltrating process. Girl, left side of brain, middle cerebral artery territory. Boy in frontal region, midline area. Thus both skin lesions associated with infiltrating process in brain. Came into clinic months after lesions started, and lesions still present.

Send your diagnosis to twip@twiv.tv

Contact

Send your questions and comments (email or mp3 file) to twip@twiv.tv

Subscribe

Subscribe to TWiP (free) in iTunes, by the RSS feed or by email

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler

The TWiVonauts review how the weather affects West Nile virus disease in the US, benefit of B cell depletion for ME/CFS patients, and an autoimmune reaction induced by influenza virus vaccine that leads to narcolepsy.

Links for this episode

• Virology faculty position, University of Toledo
• Weather and West Nile virus disease (AJTMH)
• B cell depletion benefits ME/CFS patients (PLoS One)
• Antibodies to influenza nucleoprotein cause narcolepsy (Science Transl Med)
• Letters read on TWiV 345

This episode is sponsored by ASM GAP - turn your science into a company

Weekly Science Picks

Dickson - Algal DMS releasing enzyme
Alan - Science of beer aging
Kathy - The Art of Science winners
Vincent - The future of Lego plastics

Listener Pick of the Week

Eric - Economics in One Lesson by Henry Hazzilit
Stephen - The Martian by Andy Weir (audiobook)

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Dr. Stephen C. Marini was educated in basic science and medicine at Hahnemann Medical College in Philadelphia where he received a M.S. in Microbiology and Immunology in 1976.  Doctoral studies followed at the University of Pennsylvania and Pacific Western University with dissertation research on avian tumor viruses accomplished at the Wistar Institute in Philadelphia.  He received his Ph.D. in Microbiology in 1989.  Vitalistic training and professional development occurred at the Pennsylvania College of Chiropractic where he received his D.C. in 1988.  He served as professor of Microbiology at the Pennsylvania College of Chiropractic from 1980-1995 and as Academic Dean from 1990-1993.  

As a vitalist trained in classical science and conventional medicine, Dr. Marini appreciates the role of energy/information on an individual's health and healing process.  He recognizes the need for a complementary, patient-centered approach to healing and health care options.  Dr. Steve incorporates this knowledge and belief into his teaching of psychoneuroimmunology and vaccination issues.  

Dr. Marini serves on the Boards of the International Chiropractic Pediatric Association (ICPA), the Holistic Pediatric Association (HPA), and the Integrated Healthcare Policy Consortium (IHPC).  For more information, please visit his website at www.marinichiroanded.com.  

Vincent, Dickson, and Daniel consider the delivery of anti-trypanosome nanobodies to the tsetse fly via a bacterial symbiont, and present a new case study.

Hosts: Vincent Racaniello, Dickson Despommier, and Daniel Griffin

Links for this episode:

• Delivery of a functional nanobody to tsetse flies (Microb Cell Fact)
• Nanobodies (Wikipedia)
• V.B. Wigglesworth (Wikipedia)
• VB Nimble, VB Quick
• Knott test
• Mazzotti reaction
• Image credit
• Letters read on TWiP 84

Contact

Send your questions and comments (email or mp3 file) to twip@twiv.tv

Subscribe

Subscribe to TWiP (free) in iTunes, by the RSS feed or by email

Host: Vincent Racaniello

Guests: Kartik Chandran, Ganjam Kalpana, and Margaret Kielian

Vincent travels to Albert Einstein College of Medicine where he speaks with Kartik, Ganjam, and Margaret about their work on Ebolavirus entry, a tumor suppressor that binds the HIV-1 integrase, and the entry of togaviruses and flaviviruses into cells.

Links for this episode

• Antibodies that protect against Sudan virus (ACS Chem Biol)
• Ebola virus entry requires NPC1 (EMBO J)
• A toggle switch for virus entry (Nat Comm)
• Imaging alphavirus exit (J Virol)
• Defects in HIV-1 unable to interact with INI1 (Retrovirol)
• Cell cycle arrest by INI1 (Mol Cell Biol)
• Video of this episode - view at YouTube

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Host: Vincent Racaniello

Guests: Wade Blair, Matt Dickson, Nicole Kallewaard-Lelay, and Ken Miller

Vincent visits Medimmune and speaks with Wade, Matt, Nicole, and Ken about why they work in industry and their daily roles in a biotechnology company.

Links for this episode

• Medimmune, LLC
• Medimmune on Twitter
• Medimmune history and products
• Video of this episode - view at YouTube

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler

The TWiV team consider how the kinase mTOR modulates the antibody response to provide broad protection against influenza virus, and explore the problems with scientific research.

Links for this episode

• Two lives intertwined (Ann Rev Imm)
• John Holland, 83
• Kinase mTOR modulates antibody response (Nat Imm)
• Drug widens immunity to flu (TheScientist)
• Concerto in B (TWiV 161)
• How science goes wrong (Economist)
• Trouble at the lab (Economist)
• Reforming science (TWiV 184)
• Letters read on TWiV 257

Weekly Science Picks

Dickson - Tesla Motors supercharger
Kathy - FASEB contest: Stand out for science
Alan - Natural History Museum virtual tour
Rich - Mauna Kea heavens
Vincent - FAA expands use of electronics on planes

Listener Pick of the Week

Peter - Score and ignore (pdf)

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Hosts: Vincent Racaniello, Alan Dove, and Kathy Spindler

Vincent, Alan and Kathy review novel approaches to preventing influenza virus infection.

Links for this episode:

• Ultraprotective influenza vaccine in mice (PNAS)
• Vectored immunoprophylaxis of influenza in mice (Nat Biotech)
• Intranasal antibody gene transfer protects agains influenza (Sci Transl Med)
• Estimating influenza associated deaths (CDC)
• Multistate HAV outbreak (CDC)
• Subscribe to Microbeworld video (YouTube)
• Save podcasting (EFF)
• Letters read on TWiV 236

Weekly Science Picks

Alan - Psychology of antivaccinationists
Vincent -  Virology on Coursera
Kathy - Meteorites through the ages

Listener Pick of the Week

Basel - Self-medication in animals
Sheryl - Noadi's art
Felicity - Survival of the Sickest by Sharon Moalem

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Hosts: Vincent Racaniello, Alan Dove, and Welkin Johnson

Vincent, Alan, and Welkin discuss how endogenous retroviruses in mice are held in check by the immune response.

Links for this episode:

•  Kuan-Teh Jeang, 54
  
• Resurrection of ERVs (Nature)
• Toll-like receptors control ERVs (Immunity)
• Retroviral danger from within (Immunity)
• Ancient viruses wreak havoc (The Scientist)
• Letters read on TWiV 218

Weekly Science Picks

Welkin - Viruses: Essential Agents of Life (review at STC)
Alan - Infinity Imagined
Vincent - Spaceship built for son

Listener Pick of the Week

Amanda - Written? Kitten!
Kevin - Protein synthesis: An epic (YouTube)
Daria - The Baltimore Scheme

Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Welkin joins the TWiV team for a discussion of HIV prophlaxis using vectored antibodies, and the influenza H5N1 virus studies in ferrets that were not redacted.

Links for this episode:

• Vectored HIV immunoprophylaxis (Nature)
• Vectored immunoprophylaxis - the Movie (YouTube)
• Adeno-associated virus (Wikipedia)
• In vitro evolution of H5N1 towards human receptor specificity (Virology)
• Endogenous viral genes non-essential in chicken (Nature)
• Rates of HIV transmission per coital act (J Inf Dis)
• TWiV on Facebook
• Letters read on TWiV 168

Vincent, Rich, Alan and Gabriel review the production of antibodies by B cells, and how high affinity antibodies are selected in the germinal centers of lymph nodes.